File tree Expand file tree Collapse file tree
Expand file tree Collapse file tree Original file line number Diff line number Diff line change 1- # Ingest workflow
1+ # Ingest workflow for rubella virus
22
33This workflow ingests public data from NCBI and outputs curated
4- metadata and sequences that can be used as input for the phylogenetic
5- workflow .
4+ metadata and sequences that can be used as input for the rubella
5+ ` nextclade ` and ` phylogenetic ` workflows .
66
77If you have another data source or private data that needs to be
88formatted for the phylogenetic workflow, then you can use a similar
Original file line number Diff line number Diff line change @@ -3,4 +3,3 @@ AB047330 1a(WHO) 1a(WHO)
33AF188704 1a(WHO) 1a(WHO)
44JF727653 1a(WHO) 1a(WHO)
55M30776 1a(WHO) 1a(WHO)
6-
Original file line number Diff line number Diff line change 11# Rubella Virus Nextclade Dataset Tree
22
33This workflow creates a phylogenetic tree that can be used as part of
4- a Nextclade dataset to assign clades to rubella virus samples based on
5- [ Rubella virus nomenclature update: 2013] [ ] .
4+ a Nextclade dataset to assign genotypes to rubella virus samples based
5+ on [ " Rubella virus nomenclature update: 2013" ] [ ] .
66
77* Build a tree using samples from the ` ingest ` output, with the following
88 sampling criteria:
99 * Force-include the following samples:
10- * genotype reference strains from Table 1 of [ Rubella virus
11- nomenclature update: 2013] [ ]
10+ * genotype reference strains from Table 1 of [ "Rubella virus
11+ nomenclature update: 2013"] [ ]
12+ * three additional vaccine-derived strains of genotype 1a that
13+ were empirically identified during construction of this dataset
1214* Assign genotypes to each sample and internal nodes of the tree with
1315 ` augur clades ` , using clade-defining mutations in ` defaults/clades.tsv `
1416* Provide the following coloring options on the tree:
@@ -25,4 +27,4 @@ a Nextclade dataset to assign clades to rubella virus samples based on
2527 changes to ` defaults/clades.tsv ` and re-run the workflow
2628* Repeat as needed
2729
28- [ Rubella virus nomenclature update: 2013 ] : https://www.who.int/publications/i/item/WER8832
30+ [ " Rubella virus nomenclature update: 2013" ] : https://www.who.int/publications/i/item/WER8832
Original file line number Diff line number Diff line change 1- # Phylogenetic workflow
1+ # Phylogenetic workflow for rubella virus
22
3- This workflow uses metadata and sequences to produce one or multiple
4- [ Nextstrain datasets] [ ] that can be visualized in Auspice.
3+ This workflow uses metadata and sequences to produce two
4+ [ Nextstrain datasets] [ ] that can be visualized in Auspice, one for the
5+ whole rubella genome, and one specific for the E1 gene.
56
67## Data Requirements
78
You can’t perform that action at this time.
0 commit comments