Title: License error: ConnectionError persists even after updating to latest version and re-applying Academic license

[zy137849833]

Checklist before submitting the issue:

• [√] The issue is strongly related to the MiXCR software
• [√] The issue can be reproduced with the most recent version of MiXCR
• [√] There is no answer to the question in the official documentation and there is no duplicate issue in the bug tracker
• Inspection of raw alignments with exportAlignmentsPretty shows that data has the expected architecture, and sample preparation artefacts are not the reason of the problem (if this is the matter of the issue)

Expected Result

MiXCR should start normally and recognize the valid Academic license (mi.license) without attempting to connect to the license server.

Actual Result

MiXCR fails with the following error even after:

• Updating to the latest version from GitHub
• Re-applying for a new Academic license
• Replacing the mi.license file
• The license file location has not changed (it worked before)

Exact MiXCR commands

#bash
/work/*/mixcr -V
# or
/work/*/mixcr align \
    --species HomoSapiens \
    --force-overwrite \
    input.fa \
    output.vdjca

MiXCR report files

Screen output: License error: ConnectionError
No report files (license check fails before any analysis starts)

Additional Information

DNS diagnosis:

$ curl -v https://cloud.milaboratory.com
* Could not resolve host: cloud.milaboratory.com

$ curl -v https://licensing.milaboratory.com
* Could not resolve host: licensing.milaboratory.com

nslookup returns NXDOMAIN for both domains.
The cluster can access GitHub normally (wget works), but cannot resolve Milaboratory license domains. Setting RES_OPTIONS with public DNS (8.8.8.8, 1.1.1.1) does not help — the internal DNS appears to be enforced.
We would like to run MiXCR completely offline on this cluster. Could you please advise on the best way to achieve this with an Academic license?

Thank you very much for your help!
Best regards,
Yang

[mizraelson]

Please contact us at support@milaboratories.com regarding this request.

[zy137849833]

Please contact us at support@milaboratories.com regarding this request.

Hi @mizraelson,

Thank you for the reply.

To clarify:
MiXCR worked fine on this HPC cluster for a long time without any changes. Suddenly we get License error: ConnectionError.

We have already:

• Updated to the latest MiXCR version
• Re-applied for a new Academic license
• Replaced the mi.license file

However, the error persists.

DNS check shows NXDOMAIN for both cloud.milaboratory.com and licensing.milaboratory.com, while GitHub works normally.

Questions:

1. What could cause this sudden License error: ConnectionError even though nothing was changed on our side?
2. Does the Academic license still require online validation, or should a valid mi.license file allow MiXCR to run completely offline?

If an offline license is required, we are ready to generate a new offline license request.

Thank you again for your help!

Best regards,
Yang

[mizraelson]

Academic license requires online validation, so having a valid mi.license file alone is not sufficient. It’s hard to say why it stopped working, as our licensing server IPs are static and have not changed. As long as you have access to the IPs below, it should work:

IPv4:
75.2.96.100
99.83.215.63

IPv6:
2600:9000:a403:55e8:f9c1:f443:773b:4192
2600:9000:a51b:2483:639d:d5a9:270f:6439