@@ -153,24 +153,24 @@ Below are some commonly used parameters in the format used in config files.
153153
154154Select how the transcriptome used for analysis should be prepared:
155155
156- - To create a reference transcriptome using an existing reference genome ` transcriptome_source = reference-guided ` (default)
157- - Use a a supplied transcriptome ` transcriptome_source = precomputed" `
158- - Gnerate transcriptome via the denovo pipeline ` transcriptome_source = denovo" `
156+ - To create a reference transcriptome using an existing reference genome ` -- transcriptome_source reference-guided` (default)
157+ - Use a a supplied transcriptome ` -- transcriptome_source precomputed"`
158+ - Gnerate transcriptome via the denovo pipeline ` -- transcriptome_source denovo"`
159159
160160
161- To run the workflow with direct RNA reads ` direct_rna = false ` (this just skips the pychopper step).
161+ To run the workflow with direct RNA reads ` -- direct_rna false` (this just skips the pychopper step).
162162
163- Pychopper and minimap2 can take options via ` minimap2_opts ` and ` pychopper_opts ` , for example:
163+ Pychopper and minimap2 can take options via ` -- minimap2_opts` and ` -- pychopper_opts` , for example:
164164
165165- When using the SIRV synthetic test data
166- - ` minimap2_opts = '-uf --splice-flank=no' `
166+ - ` -- minimap2_opts '-uf --splice-flank=no'`
167167- pychopper needs to know which cDNA synthesis kit used, which can be specified with
168- - SQK-PCS109: ` pychopper_opts = '-k PCS109' ` (default)
169- - SQK-PCS110: ` pychopper_opts = '-k PCS110' `
170- - SQK-PCS111: ` pychopper_opts = '-k PCS111' `
168+ - SQK-PCS109: ` -- pychopper_opts '-k PCS109'` (default)
169+ - SQK-PCS110: ` -- pychopper_opts '-k PCS110'`
170+ - SQK-PCS111: ` -- pychopper_opts '-k PCS111'`
171171- pychopper can use one of two available backends for identifying primers in the raw reads
172- - nhmmscan ` pychopper opts = '-m phmm' `
173- - edlib ` pychopper opts = '-m edlib' `
172+ - nhmmscan ` -- pychopper opts '-m phmm'`
173+ - edlib ` -- pychopper opts '-m edlib'`
174174
175175__ Note__ : edlib is set by default in the config as it's quite a lot faster. However, it may be less sensitive than nhmmscan.
176176
@@ -199,8 +199,8 @@ These should be prepared as described
199199The resulting JAFFAL reference files will look something like ` hg38_genCode22.fa` . The following options enable JAFFAL to find these
200200files:
201201
202- ` jaffal_genome = reference_genome_name` optional (default: ` hg38` )
203- ` jaffal_annotation = jaffal_annotation_prefix` optional (default: ` genCode22` )
202+ ` -- jaffal_genome reference_genome_name` optional (default: ` hg38` )
203+ ` -- jaffal_annotation jaffal_annotation_prefix` optional (default: ` genCode22` )
204204
205205
206206__Note__: JAFFAL is not currently working on Mac M1 (osx-arm64 architecture).
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