This process evaluates whether annotated intron boundaries match canonical splice site motifs (e.g., GT-AG) and are supported by transcriptomic data (if available). It checks for non-canonical splice sites, potential intron retention events, and the consistency of intron lengths. It can also correlate known splicing patterns from reference annotations with the new pangenome annotation.
This process evaluates whether annotated intron boundaries match canonical splice site motifs (e.g., GT-AG) and are supported by transcriptomic data (if available). It checks for non-canonical splice sites, potential intron retention events, and the consistency of intron lengths. It can also correlate known splicing patterns from reference annotations with the new pangenome annotation.